Shiga toxin-producing E. coli (STEC) testing

The Eurofins Food & Feed Testing laboratory network has expertise in detecting STEC. Products require reliable analytical methods to ensure they are free from this pathogen, which is caused by poor hygiene and sometimes responsible for severe infections. We offer a wide range of solutions to guarantee regulatory compliance and protect consumer health.

Key services

  • Trace detection
  • Quantification of E. coli (STEC)
  • PCR rapid method for expedited release testing

Eurofins Food & Feed Testing laboratories in Switzerland offer 24-hour clearance testing using a rapid PCR method. In the event of a positive result, an additional test to determine virulence may be carried out. There is currently no reliable marker for the infectivity of an isolate. Detection of the eae gene may provide an indication. Serotyping can be carried out and is relevant in Switzerland for decisions regarding product recalls.

We can also advise you on further analytical options, such as whole-genome sequencing, the storage of isolates in accordance with the Zoonoses Regulation, and the tracing of chains of infection in the context of your supplier monitoring.

Understanding E. coli STEC

STEC is naturally present in the intestinal microbiota of humans and animals. However, certain strains carry the stx gene, which is responsible for producing the Shiga toxin and potentially causing severe illness. Strains capable of causing clinical disease are often referred to as EHEC (Enterohemorrhagic E. coli). They can damage the kidneys, colon, nervous system, and brain, making them a major food safety concern.

Transmission and routes of contamination

The main sources of STEC transmission include raw or undercooked products such as:

  • Undercooked minced meat
  • Raw milk and unpasteurised dairy products
  • Raw vegetables and sprouted seeds
  • Unpasteurised juices (e.g. apple juice)
  • Contaminated water or flour

Contamination often occurs during slaughter, milking, irrigation, runoff from ruminant farms, or during food handling and processing. Drinking water can also be contaminated accidentally or due to insufficient treatment. Cattle and sheep are common asymptomatic carriers. 

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Would you like to find out more about our testing solutions, or do you need tailored analytical support? Contact us today.

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