Analytical instruments testing series

Training on LC-MS Triple Quad

Liquid Chromatography-Mass Spectrometry (LC-MS) is one of the most important analytical tools used for the identification, detection, and quantification of chemical compounds. Its high sensitivity and selectivity have enhanced its value across several industries. LC-MS is well suited for analysing large, ionic, polar, non-volatile, and thermally unstable compounds. By combining a liquid chromatograph with a mass spectrometer, LC-MS can separate compounds in a sample and identify them based on their characteristic mass-to-charge ratios.

Eurofins Environment Testing India offers a broad training programme to build knowledge of this instrument. A complete detailed description of the instrument, along with their theory and the technique required to use their accessories, is covered.

Aim

The programme aims to provide a complete know-how of the instrument. Eurofins Environment Testing India provides training on LC-MS/MS (6410/Agilent Technologies) according to the specified protocols, single quadrupole design, triple quadrupole, data acquisition and data quantisation. We also allow for a detailed discussion on general software and hardware, system evaluation, and building methods.

6-day training module:

Day 1

Introduction to LC-MS, theory of single quadrupole and triple quadrupole systems, system overview, ionisation sources and detectors, collision-induced dissociation, and solvents, buffers, and additives used in LC-MS.

Day 2

Tuning and calibration, product ion scan, MRM method, and HPLC method.

Day 3

Method development and optimisation, introduction to acquisition software, and worklists.

Day 4

Introduction to qualitative software, quantification basics, and the sensitivity and linearity of the Agilent triple quadrupole.

Day 5

Advanced quantification, explanation of sample preparation techniques and extraction techniques, including demonstrations.

Day 6

Maintenance of LC-MS and troubleshooting, covering issues related to the detector, column, fittings, pumps, mobile phase, tuning, and nebulisation.

Minimum eligibility required: Diploma or a graduate degree in science, or currently pursuing a qualification in a related field.

Advantages of the training programme

LC-MS has a wide scope in industries that manufacture or deal with drugs, dyes, food or dairy products. The training module is beneficial for individuals who are wanting to enter these industries.