Analytical instruments testing series

Training on HPLC

An outstanding opportunity is being offered by Eurofins Environment Testing India to gain exposure to the high-performance liquid chromatography technique (HPLC). A complete theoretical and practical knowledge on HPLC, its maintenance, method development and troubleshooting techniques can be gained from this training. We also cover valuable information on the modernised HPLC components, pumps and detectors.

Aim

Eurofins Environment Testing India offers a beneficial understanding and hands-on practice on Agilent HPLC Binary-1200 Series, 1200 Infinite series, HPLC Binary with fluorescence detector (RF 10 AXL, Shimadzu) and HPLC Isocratic with UV-VIS detector (LC-10 ATVPS, Shimadzu), helping you to level up in various industries. The training builds on each trainee's knowledge so that they are capable of applying the modern practices of HPLC. We teach you how to select the relevant instrumentation and columns, and interpret and troubleshoot the chromatograms.

6-day training module:

Day 1

Introduction to the basic principles of chromatography, several HPLC detectors and their applications, columns, binary and isocratic gradients, mobile phases, polarity, and retention time.

Day 2

Function of UV-Vis detectors and fluorescence detectors and their workings, calibration and applications.

Day 3

Covering the preparing test solutions, mobile phase, setup method for HPLC, and data acquisition software.

Day 4

Software quantisation, integration and report generation.

Day 5

Method development and optimisation of various chromatographic parameters.

Day 6

Understanding specific applications, prevention, care, maintenance, and trouble shooting.

Minimum eligibility required: Diploma or a graduate degree in science, or currently pursuing a qualification in a related field.

Advantages of the training programme

The training is beneficial for anyone who wants to establish a career in an industry that works with chemicals, cosmetics, oils, agriculture, or food and analytical testing laboratories, etc. Training on HPLC will open the gates to these industries as it is the most indispensable instrument used in the drug and pharmaceutical world.