qPCR probes
Precision, speed, real-time insights with Eurofins Genomics' qPCR probes
Unlock the full potential of your qPCR experiments with Eurofins Genomics' cutting-edge probe solutions. Whether you're focusing on gene expression analysis, SNP genotyping, or mutation detection, our qPCR probes are designed for high sensitivity, specificity, and accuracy.
With a wide array of dye and probe options, Eurofins Genomics offers tailored solutions that cater to diverse molecular biology applications.
What makes our qPCR probes stand out?
qPCR probes, also known as fluorescent probes, are designed to bring real-time PCR to a new level of accuracy. By combining the power of fluorescence with highly specific primers, our probes allow you to visualise and quantify Polymerase chain reaction (PCR) products as they are amplified. Choose from a variety of dye chemistries and probe types, including:
- Minor groove binder probes probes (MGB): Short probes with a minor groove binder for superior specificity, ideal for human IVD applications using TaqMan-MGB technology.
- Dual-labelled probes: Versatile hydrolysis probes for TaqMan assays, compatible with a range of instruments and dye-quencher combinations.
- Light cycler probes: Donor-acceptor probes suited for fluorescence resonance energy transfer (FRET) assays, perfect for applications requiring high-sensitivity fluorescence resonance energy transfer.
- Molecular beacons: Hairpin-shaped probes with an internally quenched fluorophore, detecting specific sequences with exceptional precision.
qPCR probe applications
Our probe-based qPCR services cater to applications that require multiplex PCR, where multiple targets are detected in a single reaction, maximising both efficiency and accuracy. Our probes provide unmatched quality for:
- Gene expression studies
- SNP genotyping
- Mutation detection
Frequently Asked Questions (FAQs)
qPCR probes are specialised fluorescent molecules used in real-time PCR to detect and quantify DNA or RNA in a sample. They enhance specificity and allow researchers to monitor the PCR reaction in real-time, making them essential for accurate quantification in genetic analysis.
What is the advantage of using probe-based qPCR over standard PCR methods?
Probe-based qPCR offers greater sensitivity and specificity than standard PCR. By using fluorescent labelled probes, it can detect and measure multiple targets in one reaction (multiplex PCR) and provides real-time quantification, improving the accuracy of gene expression and mutation analysis.
How do different probe types (such as MGB, Dual-Labelled, and Molecular Beacons) impact qPCR results?
Each probe type offers unique benefits. Minor groove binder (MGB) probes are highly specific, dual-labelled probes are versatile and compatible with most instruments, and molecular beacons provide enhanced accuracy for detecting specific sequences. The choice of probe depends on the application and the required level of sensitivity.
Which dye chemistries are compatible with Eurofins Genomics' qPCR probes?
Eurofins Genomics offers a variety of dye chemistries, including SYBR Green, TaqMan, and FRET-compatible dyes. These options enable researchers to select a dye and probe combination tailored to their instrument and application needs.
Can Eurofins Genomics' qPCR services support multiplex PCR?
Yes, our qPCR services support multiplex PCR, allowing the simultaneous detection of multiple targets in a single reaction. This capability is valuable for high-throughput testing and increases the efficiency of gene expression, SNP genotyping, and mutation detection experiments.