De novo and reference-based RNA-Seq

Eurofins Genomics offers a comprehensive range of RNA sequencing (RNA-Seq) services, specialising in both de novo and reference-based RNA-Seq analysis.

Our advanced sequencing solutions enable comprehensive transcriptome analysis for a wide range of organisms, including plants, animals, bacteria, and fungi. Whether you are working with model or non-model organisms, we provide customised experimental designs and detailed bioinformatics analysis to meet your research needs.

RNA-Seq: A powerful tool for transcriptome analysis

RNA-Seq is a cutting-edge method for studying the entire set of RNA transcripts, including mRNA, rRNA, tRNA, and other non-coding RNAs. It offers unparalleled sensitivity for measuring gene expression by sequencing cDNA libraries derived from mRNA.

Unlike static genomes, transcriptomes are dynamic, allowing RNA-Seq to uncover rare genes, profile mRNA, analyse gene expression, identify splice junctions and gene fusions, and detect novel transcripts in both coding and non-coding RNA.

Whole transcriptome analysis using RNA-Seq enables the identification of differentially expressed genes across various conditions, cell types, and treatments, providing valuable insights into gene regulation and function.

RNA-Seq services

Eurofins Genomics provides a range of RNA-Seq services, tailored to suit different research needs:

• Bacterial/fungal/animal/plant de novo RNA-seq
• Reference-based RNA-seq

Small RNA discovery and profiling

Small RNA-Seq can be used to discover novel micro RNAs (miRNAs) and other small non-coding RNAs, characterise variations such as isomiRs with single-base resolution, and analyze any small RNA or miRNA without prior sequence or secondary structure information. A variety of library preparation kits support customisable size selection to investigate any small RNAs ranging from 17 to 35 nucleotides and analyse the expression levels of both known and novel miRNAs in your sample.

Eurofins Genomics offers comprehensive small RNA-Seq on Illumina platforms. We offer customised experimental designs for small RNA-Seq across sample types, including plant, animal, and insects. Our established bioinformatics pipeline supports the analysis of small RNA-Seq data and provides end-to-end support, from experimental design and sequencing through to data interpretation and reporting.

LncRNA sequencing

Long non-coding RNA sequencing service (lncRNA-seq) is an extensive next-generation method to detect the non-protein-coding transcripts longer than 200nt. lncRNA regulates the expression of coding genes including epigenetic inheritance, polyadenylation, splicing, pre-transcription, transcription and post-transcription. The high-throughput sequencing technology of lncRNA combined with bioinformatics analysis can reveal the quantification and functional enrichment of the target transcripts, strand orientation and their regulatory relations.

Circular RNA sequencing

Circular RNAs (circRNAs) are non-coding RNAs with covalently closed loop without the 5′ cap and polyadenylation in the 3′ end. They play crucial roles in micro-RNA (miRNA) regulation, protein-protein interactions, regulation of parental gene transcription, and disease progression in living organisms.