We offer a range of transcriptome sequencing services tailored to different research needs. A variety of library preparation options are available for each application, enabling data generation that aligns with your specific project requirements.

Our transcriptome sequencing services include:

• De novo and reference-based RNA sequencing (RNA-seq)
• Small RNA sequencing 

De Novo RNA Seq

Identify the genes present in your transcriptome

De novo transcriptome sequencing provides a comprehensive analysis of expressed genes at a specific time point and under a defined physiological condition. Multiple sequencing reads are generated from each transcript and subsequently assembled to reconstruct the corresponding transcripts or unigenes. The libraries are sequenced using Illumina NextSeq/NovaSeq platforms. In addition, PacBio and Oxford Nanopore long-read sequencing can be performed to obtain full-length transcript sequences. Eurofins Genomics has developed a proprietary bioinformatics pipeline for the de novo assembly of short Illumina reads and long-read full-length transcripts, enabling accurate and cost-effective reconstruction of transcripts.

Advantages:

• Detect rare or previously unknown transcripts
• Strand specific protocol (upon request)
• Cost-efficient de novo transcriptome data

Reference based RNA Seq

Analyse gene expression responses and rely on our long-term experience

mRNA sequencing (mRNA-Seq) is widely used for gene expression analysis and the detection of alternative splicing events. Our RNA-Seq services provide a comprehensive view of all expressed transcripts within your sample. By capturing the entire transcriptome through high-throughput sequencing, RNA-Seq enables the identification of novel transcripts, novel isoforms, alternative splice variants, rare transcripts, and single nucleotide polymorphisms (SNPs)

Advantages:

• Analyse gene expression, gene expression abundance, alternative splice sites and SNPs
• Strand-specific protocol (upon request)
• Obtain comprehensive view of the entire transcriptome
• Analyse prokaryotic transcripts by rRNA depletion