Sample requirement

• Submit 1-3 µg of RNA-free meta genomic DNA (NGS grade) of high molecular weight, NanoDrop A260/280 ratio >1.8; A260/230=2.0-2.2; and at a concentration of ~50 ng/µl.
• Alternatively, soil, plant/ human microbiome, water or sludge samples, shipped in cool packs to our facility.

Sample quality control

• RNA free NGS grade genomic DNA (gDNA) isolation will be carried out using commercially available DNA isolation kit.
• Quality check will be carried out using agarose electrophoresis and Nanodrop. Quantification will be carried out using Qubit 3.0 Fluorometer.

Library preparation

• Amplicon optimisation will be carried out before sequencing library preparation.
• Sequencing ready customised amplicon library will be prepared and indexed using Nextera XT Index Kit of Illumina.
• Library validation will be carried out using Agilent 4200 TapeStation kit.

Sequencing

Sequencing will be carried out on Illumina MiSeq platform using 2x300 bp paired end sequencing chemistry.

Deliverable

• Raw data will be available for download as a compressed archive of FASTQ files for each sample.
• Comprehensive compiled report and data set will be shared through secure file-sharing platform.

Turnaround time

5-7 weeks from receipt of samples and all required project information. Turnaround time may vary depending on project scope, data volume, technology selected, number of samples, and overall project complexity. The estimated timeline assumes no unforeseen biological or technical challenges during sample processing.