Whole bisulfite analysis

Methylation of DNA at C5 position of cytosine impacts various cellular processes involving gene expression and chromatin remodelling. These plays a vital role in processes associated with health and development through methylation of promoter regions, cell differentiation, remodelling of chromatin, transposon silencing, etc. Eurofins Genomics offers comprehensive whole genome bisulfite analysis which is an effective method to analyse methylation of whole genomes. Eurofins Genomics has built up a proprietary pipeline to provide cost-efficient methods to customers.

RRBS – gene methylation

Reduced representation bisulfite sequencing (RRBS) is an efficient method which enables analysis of DNA methylation on genome-scale at single-nucleotide level. Focusing on the genome containing CpG dinucleotides reduces DNA sequencing cost than whole genome. RRBS sequencing combined with bioinformatics analysis provides high resolution DNA methylation detection. 

ExomeSeq analysis

ExomeSeq analysis at Eurofins Genomics represents the pinnacle of medical exome sequencing, offering unparalleled coverage and depth for gene analysis. Our ExomeSeq service delivers more than 97% coverage of 22,000 genes, with a mean read depth of 100X, ensuring that nearly all disease-associated genes are thoroughly examined. With up to 100% coverage (depth coverage ≥20X) of all exons, our ExomeSeq service doubles the gene coverage offered by many competitors, making it the most comprehensive exome sequencing solution available.

The human exome, encompassing the entire coding (exonic) region of the genome, comprises only 1-2% of the genome but contains approximately 85% of all known disease-causing variants. While traditional exome kits cover around 92% of the exome, our ExomeSeq service goes further, enabling clinicians and researchers to uncover previously unrecognised genetic causes of disease with greater precision.

Our ExomeSeq analysis can be performed for individuals (proband), trios (patient and parents), and larger family groups. This flexible approach enables targeted investigation of disease-associated genes linked to a patient's phenotype, supporting accurate diagnosis and research.

Genotyping by sequencing (GBS)

GBS is a method for high throughput single nucleotide polymorphism (SNP) discovery and also genotyping in large volume of DNA samples. This method is basically a restriction enzyme-mediated complexity reduction followed by sequencing using Illumina platforms to discover random markers across an entire genome.

Eurofins Genomics offers comprehensive sequencing on Illumina platform. We offer customised experimental design of GBS for association and genotyping studies etc. We have developed standard pipeline for advanced bioinformatics analysis of sequencing data and we provide support of experienced scientist for end-to-end analysis.

Biological database development

At Eurofins Genomics, we offer specialised and customisable database development services tailored to manage and organise genomic and clinical datasets.

Our expertise spans various applications, including whole genome sequencing, transcriptomics, small RNA analysis, exome sequencing, and more. Whether you’re handling a large dataset or planning to generate one, our databases ensure your research data is stored in an organised, accessible, and secure manner.

Advantage of biological database over traditional data storage

• Data will be stored in an organised and representative manner.
• Data can be easily added, updated or removed.
• Meaningful information can be extracted within a fraction of a second.

NCBI data submission services

Ready to share your valuable research data with the global scientific community? Eurofins Genomics offers streamlined, reliable National Centre for Biotechnology Information (NCBI) data submission services, ensuring your data becomes part of the NCBI database, a crucial step for publication and accessibility within the scientific community. From sequencing data to whole-genome projects, we support your research through every step of the submission process.

Types of NCBI submissions we support

• Bioproject: BioProjects are collections of biological data that group various data points from a single research initiative or coordinated consortium. They serve as comprehensive entry points into diverse datasets, streamlining access to related data in the NCBI database.
• Biosample: This database stores descriptions and metadata for biological source materials used in experiments, offering essential context that supports reusability, integration, and new insights from existing data.
• Sequence read archive (SRA): SRA captures sequence data from high-throughput platforms, available in aligned or unaligned formats, providing a repository for raw reads from next-generation sequencing (NGS) technologies.
• Whole genome shotgun (WGS) submissions: These submissions represent genome assemblies of incomplete genomes, supporting data for both prokaryotic and eukaryotic chromosomes sequenced using a whole-genome shotgun approach. We handle split and gapped formats, ensuring that your genomic data is properly structured.
• Transcriptome shotgun assembly (TSA): TSA stores computationally assembled transcriptome data, assembling overlapping reads into transcripts using computational methods rather than traditional cloning, ensuring efficient data sharing for transcriptomic studies.

Requirements for NCBI submission

• Submission forms: Simple forms provided by Eurofins Genomics make data organisation straightforward.
• Data formats: We support multiple formats, including Sequence Read Archive (SRA) raw data (FASTQ and FASTA) for sequencing reads and FASTA files for whole-genome sequencing (WGS) and Transcriptome Shotgun Assembly (TSA) assemblies.