High-quality replacements from Yakima Yellow and ATTO-TEC

Yakima Yellow and ATTO-TEC fluorescent dyes are excellent alternatives for VIC, NED, PET, and LIZ dyes from ABI. These dyes are suitable for various applications, including:

• Multiplex PCR
• Fragment length analysis
• STR and microsatellite analysis
• Quantitative real-time PCR

ATTO 550 - replacement for NED

• Absorption (Abs): 554 nm
• Emission (Em): 576 nm
• Extinction coefficient (EC): 120,000
• Molecular weight (MW): 691 g/mol
• Modification: NHS-Ester
• Optimal excitation range: 540-565 nm

ATTO 565 - replacement for PET

• Absorption (Abs): 563 nm
• Emission (Em): 592 nm
• Extinction coefficient (EC): 120,000
• Molecular weight (MW): 608 g/mol
• Modification: NHS-Ester
• Optimal excitation range: 545-575 nm

ATTO 633 - replacement for LIZ

• Absorption (Abs): 629 nm
• Emission (Em): 657 nm
• Extinction coefficient (EC): 130,000
• Molecular weight (MW): 649 g/mol
• Modification: NHS-Ester
• Optimal excitation range: 610-645 nm

Yakima Yellow - replacement for VIC

• Absorption (Abs): 530 nm
• Emission (Em): 549 nm
• Extinction coefficient (EC): 84,000
• Molecular weight (MW): 654 g/mol
• Modification: Phosphoramidite/NHS-ESTER
• Optimal use: Ideal for FRET-based detection systems