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Ensuring Liquid Gold is the Real Deal

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Innovative methods for fraud detection in honey

As consumer demand for natural sweeteners like honey continues to increase, so too do instances of fraud or fraud attempts by dishonest suppliers. In Europe, for instance, the European-Commission–led initiative “From the Hives” confirmed that a significant percentage of imported honey products do not comply with EU purity benchmarks. To protect consumers and legitimate domestic producers, Eurofins companies have developed a suite of state-of-the-art authentication methods to distinguish syrup-based alternatives from genuine honey.

Honey is widely regarded as one of the most adulterated foods in the world and testing to determine its authenticity is therefore extremely important. Authorities across the globe are facing rising fraud attempts, this can be seen in New Zealand through the first “fake Manuka honey” prosecution trial in 2019, or the action of the European Union to launch its “From the Hives” initiative in 2021, to assess the prevalence of honey adulterated with sugars on the EU market.

The composition and characteristics of honeys are subject to broad variation due to the numerous honey varieties and origins worldwide. This makes the reliable and decisive detection of possible fraudulent sugar syrup addition particularly difficult. In addition, fraudsters continue to push limits, and refined syrups, which often perfectly mimic honey composition, are also added by producers. These factors mean that particularly sensitive and reliable complementary testing methods are required to protect brands and customers. Eurofins has established the most advanced and reliable testing method for honey authenticity.

In 2018, Eurofins Food Integrity Control Services GmbH was the first laboratory worldwide to offer authenticity analysis of honey by liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS) as an ISO 17025 accredited method. The method enables the detection of known as well as unknown honey adulterants (targeted and untargeted analysis) as part of just one analytical test and has a significantly higher sensitivity for foreign sugar adulterants compared to previously established honey authenticity testing methods. The method combines a number of single detection methods that test for specific additives or adulterations into one multi-method which can simultaneously detect different types of sugar syrups used for adulteration, making testing more efficient and cost-effective. The Eurofins LC-HRMS method builds on the Group’s established reputation as a leader in authenticity testing for honey.

In 2014, the Eurofins Laboratory in Nantes had already pioneered a new analytical approach to test honey integrity. Developed as part of a collaborative research project, this holistic method, which uses high resolution Nuclear Magnetic Resonance (NMR), provides a wide range of information that is both targeted (quantification of defined substances) and non-targeted (identifying deviations from reference spectra). It can simultaneously detect the addition of sugars from any source, or other irregularities in the honey, such as excessive heat treatment or fermentation, and also confirm the botanical and geographical origin, independently from conventional pollen microscope analysis. An example of such advanced origin control is the control of Manuka honey authenticity by NMR. Eurofins' optimal offer for honey is a combination of these advanced methods with essential conventional ones such as pollen microscopic analysis.

The science behind

The LC-HRMS testing method is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography with the capabilities of high resolution mass spectrometry to analyse and quantify compounds, determine elemental compositions, and identify unknowns. Given the extreme sensitivity of the LC-HRMS testing method, it can largely narrow down possible chemical formulas of the unknown compounds. LC-HRMS can be used as a multimethod with hundreds of identified marker substances for the simultaneous detection of additions of different types of sugar syrups to honey. Combined with 1H NMR-Profiling, 13C stable isotope analysis and pollen analysis, it is the most advanced and reliable testing method for honey authenticity.

NMR profiling is a technique used to identify the characteristics of food – its authenticity, origin and stability – by determining specific individual profiles for food product types, like fingerprints. Eurofins carries out NMR profiling based on its worldwide database of honey (fingerprints), which comprises over 30,000 reference samples, from over 130 different botanical families. These reference samples have been collected from local producers in more than 65 countries across the world.