Quantitative PCR (qPCR) is a powerful technique used to amplify and quantify DNA or RNA sequences for a wide range of molecular biology applications. To keep up with clients’ needs and scientific innovations, Eurofins Pharma Bioanalytics Services now offers qPCR as a new service powered by the KingFisher Flex for automated nucleic acid extraction and the QuantStudio 7 Flex for high-precision qPCR analysis. This service is designed to offer a streamlined end-to-end workflow, providing fast, accurate, and reproducible results, compliant with Good Laboratory Practices (GLP), Good Clinical Laboratory Practices (GCLP), and Clinical Laboratory Improvement Amendments (CLIA).
The KingFisher Flex automates the extraction of high-quality DNA and RNA from various biological sample types, ensuring consistency and minimising manual errors, which is crucial for downstream applications. The KingFisher Flex can process up to 96 samples per run, enabling rapid and scalable sample preparation and nucleic acid extraction.
Following nucleic acid extraction, the QuantStudio 7 Flex system takes over for the qPCR analysis for a wide range of applications, including gene expression studies, single nucleotide polymorphisms (SNP) genotyping, and pathogen detection, biodistribution, viral shedding among others. The system’s versatility and precision support both simple and complex assays, including multiplexing for simultaneous analysis of multiple targets in a single reaction. It also accommodates 96- and 384- well plates, offering scalability for various project sizes.
Eurofins Pharma Bioanalytics Services’ qPCR service provides comprehensive support, from sample preparation through data analysis, ensuring a seamless and efficient process. Rigorous quality control measures are employed at every stage to ensure the highest level of accuracy and reliability in the results, adhering to GLP, GCLP, and CLIA guidelines. By leveraging the automation of KingFisher Flex and the advanced capabilities of QuantStudio 7 Flex, Eurofins Pharma Bioanalytics Services can offer fast turnaround times, scalable solutions, and cost-effective services for molecular biology needs in research, pharmaceutical, and biotechnology industrial applications.