Reliable viral sequencing begins with analytically validated methods.

Sanger sequencing remains a trusted approach for monitoring viral genetic variation and supporting infectious disease research. This white paper presents the analytical validation of a Sanger sequencing assay for the respiratory syncytial virus (RSV) NS2 gene using nasal swab specimens, demonstrating robust performance for detecting and characterizing viral mutations across both RSV A and RSV B strains.

Key topics include:

• Analytical validation of an RSV NS2 gene Sanger sequencing assay
• Performance evaluation using nasal swab specimens from RSV A and RSV B
• Assessment of analytical sensitivity, precision, accuracy, and limit of detection
• Applications of Sanger sequencing for viral mutation monitoring and infectious disease research

The validated assay demonstrated reliable sequencing performance, reproducibility, and accuracy across both major RSV groups, supporting its use for viral characterization in research and clinical settings. Learn how analytically validated sequencing methods can provide dependable data for monitoring viral evolution and advancing antiviral research.