Understand how ELISpot assay specificity supports reliable cellular immune response analysis.

ELISpot assays are widely used to measure antigen-specific T-cell responses because of their sensitivity, quantitative performance, and ability to detect low-frequency immune cells. This webinar explores the principles of assay specificity and highlights practical strategies for designing, validating, and interpreting ELISpot assays to generate meaningful immunogenicity data.

Topics include:

• How ELISpot assays measure antigen-specific T-cell activation and responsiveness
• Why ELISpot offers high sensitivity for detecting low-frequency immune responses
• Best practices for establishing and verifying assay specificity
• Approaches for evaluating and interpreting antigen-specific responses
• Key considerations when developing and validating ELISpot assays for clinical research

As cellular immunology continues to play a growing role in vaccine, cell therapy, and gene therapy development, well-designed ELISpot assays are essential for generating reliable, reproducible data. Discover practical approaches for improving assay specificity and confidence in cellular immune response measurements.