Discover key considerations for optimizing urine-based qPCR assays to support vector shedding, biodistribution, and infection identification studies.

This scientific poster highlights the evaluation of critical variables that influence the performance of urine bioanalysis methods used in gene therapy and vaccine development programs. The study assessed collection methods, storage conditions, donor variability, and automated nucleic acid extraction platforms to identify approaches that improve assay recovery, precision, and reliability for qPCR-based viral detection.

Key highlights include:

• Evaluation of urine collection methods and their impact on viral recovery and assay performance
• Comparison of automated extraction platforms, including easyMAG and KingFisher workflows, for qPCR-based bioanalysis
• Assessment of sample stability following ambient and frozen storage conditions
• Investigation of donor-to-donor variability and its effect on assay reproducibility
• Identification of optimized collection and extraction approaches that support improved recovery, precision, and data quality
• Practical insights for supporting vector shedding, biodistribution, and infection identification studies in regulated clinical research

Learn how optimized urine collection and extraction strategies can enhance qPCR assay performance, reduce variability, and support the generation of reliable bioanalytical data for gene therapy, vaccine, and infectious disease clinical programs.