Discover validated PCR methods for monitoring viral safety in cell and gene therapy.

Replication competent retroviruses (RCR) and replication competent lentiviruses (RCL) are important safety considerations during cell and gene therapy development. This poster highlights the development and validation of quantitative PCR (qPCR) assays designed to detect RCR and RCL sequences in therapeutic T cells, supporting sensitive and reliable viral safety monitoring throughout clinical development.

Key highlights include:

• Development and validation of qPCR assays for RCR and RCL detection
• Targeting of GALV envelope and VSV-G sequences to support viral safety assessments
• Evaluation of assay accuracy, precision, specificity, linearity, and dynamic range
• Sensitive detection of vector-related sequences in therapeutic T cells isolated from PBMCs
• Applications for supporting viral safety monitoring during cell therapy manufacturing and clinical trials

As cell and gene therapies continue to evolve, robust molecular assays are essential for monitoring vector safety and supporting regulatory expectations. Learn how validated qPCR methods can provide reliable detection of replication competent viral sequences throughout the clinical development lifecycle.