Harmonizing qPCR and dPCR Readouts for Standardized Analytical Use

Explore strategies for standardizing qPCR and digital PCR (dPCR) data to support consistent analytical decision-making across molecular testing workflows.

This scientific poster examines how harmonized reporting, negative control-based thresholding, and multiplex digital PCR can improve cross-platform comparisons between qPCR and dPCR. Using SARS-CoV-2 as a model, the study evaluates analytical sensitivity, precision, and quantitative performance while demonstrating practical approaches for method transfer and standardized reporting.

Key highlights include:

• Direct comparison of qPCR and Thermo Fisher Absolute Q dPCR performance using identical sample extractions
• Evaluation of sensitivity, precision, and quantitative agreement across multiple target concentrations
• Implementation of negative control-anchored thresholding to reduce false positive results
• Standardized copies/µL reporting to support consistent cross-platform method comparisons
• Insights into multiplex dPCR performance and considerations for assay selection and transfer

Learn how standardized reporting strategies and harmonized analytical workflows can improve data consistency, facilitate method transfer, and support reliable molecular testing across qPCR and digital PCR platforms.