Explore how fluorophore selection can influence qPCR assay performance and analytical sensitivity.

This technical note compares SUN™ and VIC™ fluorescent probes for quantitative PCR using identical assay designs targeting the beta-actin (ACTB) gene. Learn how probe chemistry affects amplification efficiency, signal intensity, and cycle threshold (Ct) values, and discover why SUN probes demonstrated improved performance while maintaining excellent linearity and reproducibility.

Key topics include:

• A direct comparison of SUN™ and VIC™ fluorescent probes in qPCR assays
• The impact of probe selection on amplification efficiency, signal intensity, and Ct values
• Evaluation of assay linearity, reproducibility, and quantitative performance across serial dilutions
• Practical considerations for selecting fluorescent probes to optimize qPCR assay performance

As qPCR continues to play a critical role in drug development and molecular testing, optimizing assay components can improve analytical performance and confidence in quantitative results. Discover how fluorophore selection can enhance qPCR sensitivity and overall assay performance.