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Cell Based Phenotypic >> Oncology >> OncoPanel 3D

OncoPanel™ 3D – determine activity of compounds on tumor spheroids

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OncoPanel™ 3D is a drug profiling platform comprised of ~100 cancer cell line models grown as three-dimensional (3D) tumor spheroids that phenocopy key attributes of human tumors. Drug testing is initiated only after spheroid formation has been confirmed through hypoxia-positive spheroid calling using high-content imaging. This platform provides a means to compare the tumor penetrating properties of a collection of drugs by comparing activity in 2D versus 3D cell culture.

Advantages of OncoPanel™ 3D:

  • Largest commercial panel of 3D cancer cells with ~100 cell lines
  • 3D culture phenocopies morphological and phenotypic characteristics of in vivo tumors
  • Comparisons of 2D vs. 3D drug responses can identify molecules that display enhanced tumor penetration
  • Cultures optimized to ensure >80% of cells exist within spheroids or loose aggregates at the time of treatment
  • The homogenous, luminescense-based assay, CellTiterGLO® 3D is used to analyze drug response 


CELL TYPE # of Cell Lines
Bladder 6
Breast 6
Central Nervous System 7
Colon 15
Endocrine 1
Female GU 14
Head and Neck 8
Kidney 5
Liver 5
Lung 11
Melanoma 1
Pancreas 7
Prostate 3
Skin 7
Soft Tissue 8


Figure 1. Comparing drug response between 2D vs. 3D culture

Top) Representative bright field and LOX-1, hypoxia-sensitive dye, fluorescent images of WiDr and NCI-H460 cells demonstrating that the majority of cells exist in spheroids.  Bottom) Dose response curves for four inhibitors with distinct mechanisms of action generated after 7 days of treatment either in 2D or 3D cell culture. Note, the difference between these two culture conditions is more pronounced, in general, for NCI-H460 cells than for WiDr cells with some, but not all, inhibitors.