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Bioanalysis >> Biomarker Testing Services >> Qualified Flow Cytometry Immunophenotyping Assays

Qualified Flow Cytometry Immunophenotyping Assays for Use in Clinical Trials

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Panels for T-cell, B-cell and NK cell (TBNK) for the detection of T cells including the CD4 and CD8 subsets, B cells, NK cells, NKT cells lymphocytes and monocytes, and T-helper (Th) which identifies several key functional T lymphocyte subsets (Th1, Th2, Th17) have been developed and partially validated. These assays show suitable precision between donors and runs, including for lower frequency cell populations such as Th17 cells


Markers detected by the TBNK and Th panels

  BV421 BV510 Ax488 PE PerCP
PECy7 Ax647 APC-Cy7
TBNK CD3 Fix L/D Aqua CD45 CD14 CD4 CD19 CD16 /
Th  CD3 Fix L/D Aqua - CD183 CD4 CD196 CD161 CD8


Assay Characteristics

Validated with both whole blood and cryopreserved PBMCs

Qualified for use with several commonly used anti-coagulants

Reproducibility with low event #

                1000 events for the TBNK panel

                5000 lymphocyte events for the Th panel

Good inter- and intra-assay precision

Cross-site validated at both Eurofins Bioanalytical Services facilities